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KMID : 0545120180280030401
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 3 p.401 ~ p.408
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Enhancement of Ethanol Production via Hyper Thermal Acid Hydrolysis and Co-Fermentation Using Waste Seaweed from Gwangalli Beach, Busan, Korea
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Sunwoo In-Yung
Nguyen Trung Hau
Sukwong Pailin
Jeong Gwi-Teak
Kim Sung-Koo
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Abstract
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The waste seaweed from Gwangalli beach, Busan, Korea was utilized as biomass for ethanol production. Sagassum fulvellum (brown seaweed, Mojaban in Korean name) comprised 72% of the biomass. The optimal hyper thermal acid hydrolysis conditions were obtained as 8% slurry contents, 138 mM sulfuric acid, and 160oC of treatment temperature for 10 min with a low content of inhibitory compounds. To obtain more monosaccharides, enzymatic saccharification was carried out with Viscozyme L for 48 h. After pretreatment, 34 g/l of monosaccharides were obtained. Pichia stipitis and Pichia angophorae were selected as optimal co-fermentation yeasts to convert all of the monosaccharides in the hydrolysate to ethanol. Co-fermentation was carried out with various inoculum ratios of P. stipitis and P. angophorae. The maximum ethanol concentration of 16.0 g/l was produced using P. stipitis and P. angophorae in a 3:1 inoculum ratio, with an ethanol yield of 0.47 in 72 h. Ethanol fermentation using yeast co-culture may offer an efficient disposal method for waste seaweed while enhancing the utilization of monosaccharides and production of ethanol.
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KEYWORD
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Waste seaweed, hyper thermal acid hydrolysis, enzymatic saccharification, co-fermentation
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